Overexpression of Alternatively Spliced Tissue Factor Induces the Pro-Angiogenic Properties of Murine Cardiomyocytic HL-1 Cells
スポンサーリンク
概要
- 論文の詳細を見る
Background: Tissue factor (TF) is expressed in 2 isoforms: membrane-bound "full length" (fl)TF and soluble alternatively spliced (as)TF. flTF is the major thrombogenic form of TF. Although the function of asTF is poorly understood, it was suggested that asTF contributes to tumor-associated growth and angiogenesis. In the heart of a developing embryo, asTF is expressed much later compared to flTF, but in adult heart, asTF exhibits a distribution pattern similar to that of flTF. Thus, it is possible that asTF may play a role in heart development via pro-angiogenic signaling. The purpose of the present study was to examine the effects of murine asTF overexpression in murine cardiomyocyte-like HL-1 cells on their pro-angiogenic potential, the chemotaxis of monocytic cells, and the expression of fibroblast growth factor-2 (FGF2), cysteine-rich 61 (Cyr61), and vascular endothelial growth factor (VEGF). Methods and Results: Expression of FGF2, Cyr61 and VEGF was assessed on reverse transcription-polymerase chain reaction and western blot. Cell migration, proliferation, and endothelial tube formation assays were carried out. It was found that overexpression of murine asTF in HL-1 cells increases their proliferation and pro-angiogenic properties. The supernatant of murine asTF-overexpressing HL-1 cells induces the chemotaxis of monocytic cells. Conclusions: Overexpression of murine asTF in murine cardiomyocytic cells increases their proliferation, monocyte migration, and pro-angiogenic properties -possibly- mediated by the induction of the pro-migratory and pro-angiogenic factors FGF2, Cyr61 and VEGF. Thus, we propose that murine asTF may serve as a migration- and angiogenesis-promoting factor. (Circ J 2011; 75: 1235-1242)
論文 | ランダム
- 215 子宮癌、乳癌検診受診者における甲状腺穿刺吸引細胞診の成績(甲状腺III)(示説演題)(第31回日本臨床細胞学会秋期大会学術集会)
- 集団遊びの発展の条件と指導について : 集団機能および役割の分化を中心として
- 7 乳腺粘液癌の3例(乳腺II)(示説演題)(第31回日本臨床細胞学会秋期大会学術集会)
- エンドサイト法による子宮内膜細胞診の検討
- 318.卵巣のEndodermal sinus tumorの1症例(婦人科19:卵巣III, 示説, 第27回日本臨床細胞学会秋期大会学術集会)