A New Serotype-Specific Antigen, Designated Serotype g, from Actinobacillus Actinomycetemcomitans

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概要

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• During the isolation of Actinobacillus actinomycetemcomitans from periodontal pockets, a serologically untypeable strain, designated NUM-Aa 4039, was observed. Biochemical and serological identification and PCR-based analysis confirmed that NUM-Aa 4039 was a strain of A. actinomycetemcomitans. An antigen from NUM-Aa 4039 did not react with a to e serotype-specific antibodies in double-immunodiffusion experiments. DNA from the strain could not be amplified by PCR using specific PCR DNA primer of a to f serotype-specific O-polysaccharide gene cluster. The antigen was extracted from whole cells by autoclaving and purified by ion-exchange and gel filtration chromatography. The molecular weight was approximately 180,000. The new antigen was composed of glucose, rhamnose and N-acetyl-glucosamine. Double immunodiffusion and ELISA experiments with antigens and antibodies showed that the antigen had individual antigen determinant. I propose that untyped strain be assigned to a new A. actinomycetemcomitans serotype, designated serotype g.

• 日本大学松戸歯学部 口腔科学研究所の論文

著者

• Saito Masanori

  Nihon University Graduate School of Dentistry at Matsudo, Clinical Pathology

関連論文

• A New Serotype-Specific Antigen, Designated Serotype g, from Actinobacillus Actinomycetemcomitans

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