Reduced Dental Caries Susceptibility in Chromosome 2-Substituted Consomic Mice

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In this study, to clarify the effects on dental caries susceptibility of mouse chromosome 2 in vivo, we used chromosome 2 substitution mice (termed consomic mice), B6-Chr2C3H. The consomic mice used in this study were based on high dental caries susceptibility strain C57BL/6CrSlc (B6), and only chromosome 2 from the low dental caries susceptibility strain C3H/HeSlc (C3H) was substituted. We induced dental caries in three inbred mice strains : B6, C3H and B6-Chr2C3H. All mice were weaned at 21 days, were fed on Diet#2000, and were infected with Streptococcus mutans JC-2 to induce dental caries. The mandible bones were extracted at 49 days and observed by microscope and Micro-focus X-ray computed tomography images. The caries status was evaluated by calculating the dental caries score using the modified Keyes method. Mean dental caries scores of B6-Chr2C3H, B6 and C3H were 8.1±3.4 (mean±S.D.), 46.5±1.3 and 2.2±1.0, respectively. Statistical analysis revealed that the dental caries score of B6-Chr2C3H was significantly low compared with the score of B6 and there was no difference in dental caries score between C3H and B6-Chr2C3H. The first fissures of the first molar in B6 and B6-Chr2C3H were similar having a slight fossa at center area and which were differ from that of C3H. However, there was no significant difference in dental caries score at the fissure among B6-Chr2C3H, B6 and C3H. And, the amount of saliva of B6-Chr2C3H was significantly low than C3H, and significantly high than B6. These results suggested that the major genetic factor responsible for dental caries susceptibility is located on mouse chromosome 2.

Reduced Dental Caries Susceptibility in Chromosome 2-Substituted Consomic Mice

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