Expression of Desmosomal Proteins in Rat Keratinocytes during In Vitro Differentiation
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The keratinocyte, the major component of the epidermis, expresses several proteins that characterize the keratinization during the differentiation. Proliferation and differentiation of cultured human keratinocytes are known to be regulated by the Ca2+ concentration in the culture medium. However, informations about the rat keratinocyte are relatively limited and their physiology is still an open question. To elucidate the characteristics of the rat keratinocyte, we established rat keratinocyte culture system and examined effects of extracellular calcium concentration on the expression of differentiation-related proteins. Keratinocytes were isolated from the newborn rat skin with 0.25% trypsin, followed by separation with a Percoll density gradient. The separated cells were grown in MCDB153 medium containing several growth factors and Ca2+-free fetal bovine serum, then stimulated with Ca2+. Immunoblotting demonstrated strong expression of β1 integrin in unstimulated cells, suggesting that the primary culture of rat keratinocytes was successfully established. Expression of desmoglein and transglutaminase was increased by Ca2+ stimulation, whereas β1 integrin expression was decreased in response to increasing concentrations of Ca2+. These observations indicate that cultured rat keratinocytes maintain the ability to differentiate in vitro, which is similar to that of the basal keratinocytes in the epidermis.
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- 戦前日本の統制経済論とドイツ経済思想--資本主義の転化・修正をめぐって
- 戦前日本の統制経済論とドイツ経済思想 : 資本主義の転化・修正をめぐって
- 西ドイツにおける戦後経済改革 : ヨーロッパの戦後改革の一環として(第3セッション 戦後改革50年,20世紀資本主義-歴史と方法の再検討-,創立五十周年記念大会報告集)
- 馬場 哲著, 『ドイツ農村工業史 : プロト工業化・地域・世界市場』, 東京大学出版会、一九九三年一二月、vi+二六五頁、五九七四円
- 田村信一著, 『グスタフ・シュモラー研究』, 御茶の水書房, 1993年, vii+395頁+人名策7