ラット腱板断裂モデルを用いた腱板断裂後筋組織内脂肪浸潤の分子生物学的解析
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It has been reported that mechanical stretch increases the gene expression of Wnt10b and decreases adipogenic differentiation of myogenic cells in vitro. We investigated the gene expression of Wnt10b and markers for fatty infiltration in the torn rotator cuff muscle using a rat model. Twenty-four Sprague-Dawley rats were used for the current study. Right supraspinatus and infraspinatus tendons were detached from the greater tuberosity (cuff-tear side). Sham operation was performed in the left shoulders, which served as controls (control side). The rats were euthanized at 3, 7, 14, and 28 days after the surgery. Total RNA was extracted from the proximal and distal parts of the supraspinatus muscles. Then, quantitative PCR reactions were performed to determine the gene expressions of Wnt10b and the adipogenic markers, PPAR γ and C/EBP α. The expression profiles were compared between the cuff-tear and control sides. Fatty infiltration was confirmed by Oil red O staining at 56 days after the surgery. Decreased expression of Wnt10b in the cuff-tear side was observed at 3 and 7 days in the distal part and at 3 days in the proximal part. PPAR γ mRNA expression exhibited significant increase in the cuff-tear side at 28 days. C/EBP α gradually increased in the cuff-tear side. Oil red O positive fatty infiltration was observed at the musculotendinous junction only in the cuff-tear side. Torn rotator cuff muscles showed decreased gene expression of Wnt10b. The reduction of Wnt10b might play a role in the molecular mechanism of the fatty infiltration into the supraspinatus muscle.
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