Ascorbic Acid Enhances Radiation-induced Apoptosis in an HL60 Human Leukemia Cell Line
スポンサーリンク
概要
- 論文の詳細を見る
This study was conducted to examine the utility of the combined use of ascorbic acid (AsA) and radiation in clinical applications. We investigated cell survival, DNA fragmentation, and caspase activation after X-ray irradiation and AsA treatment of human leukemia HL60 cells. The number of living cells decreased after combined X-ray irradiation and AsA treatment (2 Gy + 5 mM) in comparison with that after X-ray irradiation (2 Gy) or AsA treatment (5 mM) alone. DNA fragmentation was more in the cells subjected to combined X-ray irradiation and AsA treatment than in those subjected to X-ray irradiation alone. Caspase-3, caspase-8, and caspase-9 were highly activated following combined X-ray irradiation and AsA treatment, but caspase-8 activity was not markedly increased after X-ray irradiation alone. Bax levels in the mitochondrial membrane fractions were increased after AsA treatment alone and after combined X-ray irradiation and AsA treatment. However, there was no apparent increase in the Bax levels after X-ray irradiation treatment alone. Thus, this study confirmed that supplementing X-ray irradiation with AsA treatment results in increased apoptosis in HL60 cells. With regard to the apoptosis-inducing factors, we hypothesized that Bax and caspase-8 were activated after combined X-ray irradiation and AsA treatment compared with either treatment alone.
論文 | ランダム
- 中国帰国者の見た日本-1-日本人,中国人,音楽,人生など徒然なるままに現実と私の夢--未熟音楽家の独白
- 一作曲家の考えたこと--私は何のために作曲するのか (誰のために作曲するのか?)
- 夕鶴と私たち--文学・音楽・美術と歩む子どもの本の店
- 日本人であること--私の音楽論 (作曲家はなにを考えている)
- 『右大臣実朝』と取組んで