Induction of Chlorosis, ROS Generation and Cell Death by a Toxin Isolated from Pyricularia oryzae
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概要
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The ethyl acetate extract of the conidia germination fluid from an Avena isolate (Br58) of Pyricularia oryzae had chlorosis-inducing activity on oat leaf segments. The same activity was also present in the acetone extract of an oatmeal agar culture of Br58. Fungal cultures were used for a large-scale preparation. A series of acetone and ethyl acetate extraction monitored by chromatography was used to isolate an active fraction. The active principle was purified by HPLC. We show by NMR and LC/MS that the toxin was an oxidized C18 unsaturated fatty acid named Mag-toxin. Mag-toxin induced chlorosis on oat leaf segments incubated in the light but not in the dark. Reactive oxygen species (ROS) and cell death were induced by Mag-toxin in oat cells. The sub-cellular localization of ROS generation induced by the toxin treatment was correlated with the location of mitochondria. Interestingly, the induction of ROS generation and cell death by Mag-toxin was light-independent.
論文 | ランダム
- 生体試料中のトリフェニル・スズおよび代謝物の同時微量分析法
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- 有毒ガス・蒸気吸入 (呼吸器疾患の救急治療) -- (各種病態に対する救急治療の実際)
- 高速液体クロマトグラフィ-による尿中馬尿酸・メチル馬尿酸の定量