Suppression of carotenoid synthesis in transgenic Arabidopsis cultured cells over-expressing the AHL29/SOB3 gene
スポンサーリンク
概要
- 論文の詳細を見る
Up-regulation of the Arabidopsis gene AHL29/SOB3 (At1g76500), encoding a protein with an AT-hook DNA-binding protein motif, is known to function as a suppressor of the phyB phenotype and to cause delayed senescence. We over-expressed the full-length cDNA of AHL29/SOB3 under the cauliflower mosaic virus 35S promoter in Arabidopsis suspension-cultured T87 cells. Preliminary DNA array experiments suggested down-regulation of many genes of the carotenoid synthesis pathway in the transgenic cells. Metabolite analysis with a liquid chromatography-coupled mass spectrometer demonstrated that the accumulation of the carotenoids lycopene, α-carotene, β-carotene, lutein, zeaxanthin, antheraxanthin, violaxanthin and neoxanthin was suppressed in the transgenic cells. These results suggest that up-regulation of AHL29/SOB3 down-regulates the mechanism of carotenoid biosynthesis at the transcriptional level.
論文 | ランダム
- 41486 活性炭系ケミカルフィルタの性能向上に関する研究(汚染の除去・制御,環境工学II)
- 46. 異方性3次元フーリエ変換法の基礎的検討 : MRI-1 測定技術 : 第43回総会会員研究発表予稿
- 衝撃微粒子の付着堆積現象
- 41484 低アウトガス性ケーブルの開発(カビ・汚染質発生量,環境工学II)
- ウィナスペクトルの測定について : 総合委員会公開討論会