ポリアクリルアミド系蛍光センサー

概要

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In this account, polyacrylamide-based fluorescent sensors (thermometer, logic gate, and digital ion sensor) are reviewed. Generally, these polymeric sensors equilibrate in aqueous solution between an extended form with hydration by solvent water molecules and a globular form induced by hydrophobic interaction of side chains. Water-sensitive fluorophore 4-dimethylsulfamoyl-7-aminobenzofurazan incorporated into these polymeric sensors only emitted strong fluorescence at their globular forms where water molecules as quenchers could not get close to the fluorophore. Fluorescent thermometers were based on thermo-responsive poly (N-isopropylacrylamide), poly (N-n-propylacrylamide), and poly (N-isopropylmethacrylamide). Their modifications in solubility enabled intracellular temperature measurement for the first time. A fluorescent logic gate based on a copolymer of N-t-butylacrylamide and ionizable N, N-dimethylaminopropylacrylamide fluoresced only under limited conditions (i.e., high temperature and high pH). Digital fluorescent pH sensors were based on copolymers of N-isopropylacrylamide and ionizable acrylamide derivatives. Their fluorescence off-on switching was completed by changing pH by less than one unit.