Molecular Cloning, Characterization, and Differential Expression of a Lanosterol Synthase Gene from Ganoderma lucidum
スポンサーリンク
概要
- 論文の詳細を見る
A homology-based PCR method was used to clone a cDNA encoding lanosterol synthase (LS) from Ganoderma lucidum (G. lucidum), which produces triterpenes. The cDNA of the LS (GenBank accession no. GQ169528) was found to contain an open reading frame (ORF) of 2,181 bp encoding a 726 amino acid polypeptide, whereas the LS genomic DNA sequence (GenBank accession no. GQ169529) consists of 2,924 bp. Functional complementation of G. lucidum LS (Gl-LS) in an erg7 yeast strain lacking LS activity demonstrated that the cloned cDNA encoded a functional LS. Analysis of the Gl-LS transcript profiles revealed a positive correlation between the pattern of LS gene expression and triterpenes content changes in G. lucidum during development. Up-regulation of expression of the Gl-LS gene by methyl jasmonate (MeJA) in the mycelia was also demonstrated by real-time RT-PCR. Up-regulation of the Gl-LS promoter activity by MeJA was also investigated.
論文 | ランダム
- イネいもち病防除におけるプロベナゾールのイネ体に与える影響
- 3)遅延型反応と Transfer(II 抗体の構造とその生物学的意義)
- 42.結核感作モルモット腹腔内細胞による「ツベルクリンアレルギー」の受身伝達に関する研究(G. 結核アレルギーに関するもの)(第16回日本アレルギー学会総会)
- (9) イネ白葉枯病に対するオリゼメートの効果 (昭和52年度地域部会講演要旨(秋季関東部会))
- 5. "Transter Factor"の化学(III免疫化学)(第16回日本アレルギー学会総会)