Granulocyte Colony-Stimulating Factor Treatment Prevents Cognitive Impairment Following Status Epilepticus in Rats

スポンサーリンク

概要

• 論文の詳細を見る

• Status epilepticus (SE) rendered selective neuronal loss and cognitive impairments. Previous studies proved that granulocyte colony-stimulating factor (G-CSF) acted as a neuroprotectant in some nervous diseases. However, no investigations were focused on whether G-CSF could protect the hippocampus from SE. In this study, we administered recombinant human G-CSF into Sprague-Dawley rats with lithium-pilocarpine-induced SE subcutaneously for three times. The Morris water maze was employed to determine spatial learning ability from the 15th to 20th days after the treatment. The quantitative terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) staining and levels of apoptosis-related molecules including cleaved caspase-3, Bcl-xL and Bax on hippocampal CA1 region were examined by immunohistochemical staining at the 3rd and the 5th day after the treatment. Moreover, the phosphorylation of AKT was evaluated with Western blot at the 6th, 24th and 48th hours after the treatment to explore apoptosis and detect the protective effects of G-CSF. We found G-CSF treatment prevented SE-induced cognitive impairments with the decreased escape latency time on the 17th (29.86±9.09 vs. 38.33±6.94, p<0.05) and 18th days (23.83±6.17 vs. 33.52±8.48, p<0.05). The reduced TUNEL staining demonstrated reduced neuronal apoptosis occurrences. The anti-apoptotic effects were associated with decreased cleaved caspase-3 and Bax expression and increased phosphorylation of AKT and Bcl-xL expression. Taken together, our results suggested that systemic G-CSF treatment conducted neuroprotective function following SE through an anti-apoptotic pathway and prevented cognitive impairments, which may provide novel insights into pathogenesis and treatment following SE injury.

著者

• Zhao Gang

  Department Of Cardiovascular Medicine Graduate School Of Medicine Sciences Kyushu University

• Zhang Yong

  Department Of Computer Science And Engineering Shanghai Jiao Tong University

• Wang Wenyong

  Departments of Electrical Engineering, Applied Physics, and Physics, Yale University, P.O. Box 20828

• Sun ZhiJian

  Department of Histology and Embryology, The Fourth Military Medical University

• Feng DongYun

  Department of Neurology, Xijing Hospital, The Fourth Military Medical University

• Deng YanChun

  Department of Neurology, Xijing Hospital, The Fourth Military Medical University

• Liu YongHong

  Department of Neurology, Xijing Hospital, The Fourth Military Medical University

• Wang HuaNing

  Department of Psychiatry, Xijing Hospital, The Fourth Military Medical University

• Huang YuanGui

  Department of Neurology, Xijing Hospital, The Fourth Military Medical University

• Wang Wenyong

  Departments Of Electrical Engineering Applied Physics And Physics Yale University

• Zhang Yong

  Department Of Chemistry & Chemical Engineering Suzhou University

• Zhang Yong

  Department Of Applied Bioresource Science The United Graduate School Of Agricultural Sciences Ehime University

• Zhao Gang

  Department of Neurology, Xijing Hospital, The Fourth Military Medical University

• Wang WenYong

  Department of Pathology, The Fourth Military Medical University

関連論文

• Effects of Lifestyle Factors on Stiffness Index of Calcaneus Measured by Quantitative Ultrasound System among Japanese Women Aged 40 Years and Over : The Hizen-Oshima Study
• SiO and H_2O Maser Observations of Red Supergiants in Star Clusters Embedded in the Galactic Disk
• Synergistic protecting effect of cord blood CD34^+ cells over-expressing both interleukin-3 and Flt3 ligand on lethally irradiated mice
• Anti-inflammation Strategy Via Local Gene Transfer with Geneeluting Stents Attenuates in-stent Restenosis in Hypercholesterolemic Rabbits and Monkeys(Coronary Revascularization, PTCA/Stent/DCA/Rotablator/New Device 16 (IHD), The 69th Annual Scientific Mee
• Angiotensin II AT1 Receptor-Mediated Enhanced RAGE Signals are Responsible for the Mechanism of Accerelated Atherosclerosis by Diabetes Mellitus(Atherosclerosis, Basic 10 (IHD), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
• A Well Characterized Example of Conversion of Molecular Configuration Depending on the Existence of Intermolecular Interactions
• Epigenetic and genetic alterations of PTEN in hepatocellular carcinoma
• A Fuzzy Control-Based Service Configuration Approach for Ubiquitous Computing Applications
• An On-Demand QoS Service Composition Protocol for MANETs(Networks)
• Tanshinone IIA Attenuates Seawater Aspiration-Induced Lung Injury by Inhibiting Macrophage Migration Inhibitory Factor
• Protective Effects of Ginsenoside Rd on PC12 Cells against Hydrogen Peroxide(Pharmacology)
• 2 Stent-Based Local Delivery of Nuclear Factor-k B Decoy Attenuates In-Stent Restenosis in Hypercholesterolemic Rabbits(Young Investigator's Award Finalists Lecture (YIA),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
• FRS-166 Stent-based delivery of nuclear factor-κB(NF-κB) attenuates in-stent restenosis in hypercholesterolemic rabbits(Coronary Heart Disease : Basic Science (IHD) : FRS20)(Featured Research Session (English))
• Dielectric Anomalous Peak of Relaxor Ferroelectrics under Small DC Bias
• Granulocyte Colony-Stimulating Factor Treatment Prevents Cognitive Impairment Following Status Epilepticus in Rats
• Chinese Hypertriglycerideamic Subjects of Different Ages Responded Differently to Consuming Oil with Medium- and Long-Chain Fatty Acids
• Crystallographic Analysis of {225}_f Martensite Transformation in Fe-Cr-C Alloy
• Intrinsic Electronic Transport through Alkanedithiol Self-Assembled Monolayer
• Triglyceride with Medium-Chain Fatty Acids Increases the Activity and Expression of Hormone-Sensitive Lipase in White Adipose Tissue of C57BL/6J Mice
• UV irradiation improves the bond strength of resin cement to fiber posts
• Correlation between Glass Formation and Type of Eutectic Coupled Zone in Eutectic Alloys
• Triglyceride with Medium-Chain Fatty Acids Increases the Activity and Expression of Hormone-Sensitive Lipase in White Adipose Tissue of C57BL/6J Mice
• Androgen Deprivation Induces Bladder Histological Abnormalities and Dysfunction via TGF-β in Orchiectomized Mature Rats
• Correlation between catalytic activity and monomer-dimer equilibrium of bacterial alanine racemases
• Increased Norepinephrine by Medium-Chain Triglyceride Attributable to Lipolysis in White and Brown Adipose Tissue of C57BL/6J Mice
• Medium-Chain Fatty Acids Enhanced the Excretion of Fecal Cholesterol and Cholic Acid in C57BL/6J Mice Fed a Cholesterol-Rich Diet
• Impaired activities of cyclic adenosine monophosphate-responsive element binding protein, protein kinase A and calcium-independent phospholipase A2 are involved in deteriorated regeneration of cirrhotic liver after partial hepatectomy in rats
• Rosuvastatin attenuates the elevation in blood pressure induced by overexpression of human C-reactive protein
• The role of Tα1 on the infective patients after hematopoietic stem cell transplantation
• Medium-Chain Fatty Acids Enhanced the Excretion of Fecal Cholesterol and Cholic Acid in C57BL/6J Mice Fed a Cholesterol-rich Diet

もっと見る 閉じる

スポンサーリンク