Usefulness of the Addition of Orvus ES Paste and Sodium Lauryl Sulfate to Frozen Feline Semen

概要

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It has been shown that the addition of the surfactant Orvus ES paste (OEP) and its main component sodium lauryl sulfate (SLS) to boar or dog semen before freezing improves post-thaw sperm motility and protects acrosome caps. In this study, we investigated the usefulness of the addition of OEP (0, 1, 2, and 4%) or SLS (0, 1, 2, 3, and 4 mg/ml) to cat ejaculates before freezing and their concentration. Among the OEP addition groups, the 1% OEP group showed higher sperm motility than the other groups. Among the SLS addition groups, the 3 mg/ml SLS group showed slightly higher sperm motility and viability than the other groups. Comparison between the 1% OEP and 3 mg/ml SLS addition groups suggested a higher percentage of sperm with an acrosome cap in the 1% OEP group. The other sperm properties did not significantly differ between the two groups. These results indicate that the addition of 1% OEP or 3 mg/ml SLS is effective for the freezing of cat ejaculated semen.