Myosin Motor-Like Domain of the Class VI Chitin Synthase CsmB Is Essential to Its Functions in Aspergillus nidulans
スポンサーリンク
概要
- 論文の詳細を見る
Chitin is one of the major cell wall components of ascomycete filamentous fungi, and chitin synthesis plays important roles in the morphogenesis of hyphae. In the Aspergillus nidulans genome, there are two genes, csmA and csmB, that encode a myosin motor-like domain (MMD) at their N-termini and a chitin synthase domain (CSD) at their C-termini. In our previous studies, we found that the MMD of CsmA was required for its functionality, and that CsmA and CsmB had certain overlapping functions essential for polarized filamentous growth. In this study, we constructed a strain that produced CsmB lacking the MMD (CSΔMB). This strain exhibited defects similar to those of the csmB deletion mutant. FLAG-tagged CSΔMB (CSΔMB-FLAG) did not properly localize to the hyphae. CsmB was co-immunoprecipitated with actin in vivo, whereas CSΔMB was not. These results suggest that the MMD of CsmB is crucial for its proper localization via interaction with actin.
論文 | ランダム
- 資料 青森放送の下請をめぐる労使関係の法的とらえ方にたいする意見 (青森放送事件・青森地裁判決の意義)
- 「労使関係」と人権--歪んだ労使関係 どこから正すか (労使関係と人権)
- 現代女子労働行政への提言--戦後女子労働行政の足跡・問題点とあるべき姿 (婦人の保護と平等--どう考えるか)
- 労災訴訟と労働組合--東海カ-ボン事件の判決によせて
- 管理職手当と時間外賃金--問題の背景と新通達の意義・課題 (外勤労働者・管理者の時間外労働--実情と問題点)