SLC39A9 (ZIP9) Regulates Zinc Homeostasis in the Secretory Pathway: Characterization of the ZIP Subfamily I Protein in Vertebrate Cells
スポンサーリンク
概要
- 論文の詳細を見る
The SLC39A family of zinc transporters can be divided into four subfamilies (I, II, LIV-1, and gufA) in vertebrates, but studies of their functions have been restricted exclusively to members of subfamilies II and LIV-1. In this study, we characterized SLC39A9 (ZIP9), the only member of subfamily I in vertebrates. Confocal microscopy demonstrated that transiently expressed, HA-tagged human ZIP9 (hZIP9-HA) was localized to the trans-Golgi network regardless of zinc status. Disruption of the ZIP9 gene in DT40 cells did not change the growth rate, sensitivity to high zinc and manganese concentrations during long-term culture, or cellular zinc status after short-term incubation with zinc. The alkaline phosphatase activity of ZIP9−⁄− cells did not change in cells cultured in medium containing normal zinc levels. In contrast, the activity of this enzyme decreased in wild-type cells cultured in zinc deficient medium but less so in ZIP9−⁄− cells under these conditions. Stable over-expression of hZIP9-HA moderately decreased alkaline phophatase activity. These results suggest that ZIP9 functions to regulate zinc homeostasis in the secretory pathway without significantly altering cytosolic zinc homeostasis.
論文 | ランダム
- 遮蔽物の透過率に基づく森林地帯におけるGPS電波の受信可能性
- Laparoscopic necrosectomy for acute necrotizing pancreatitis
- KPOS-JPOA exchange fellowship
- 19. 前半規管型良性発作性頭位めまい症(anterior canal benign paroxysmal positional vertigo; AC-BPPV)の臨床像と治療法(第1101回千葉医学会例会・第22回神経内科教室例会)
- 4.Venous pooling症候群を示した頸部脊椎症の1例(第818回 千葉医学会例会・第7回 神経内科例会)