Crystal Structures of Sodium-Bound Annexin A4

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概要

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• Annexin A4 (Anx4) possesses four repeat domains with one Ca2+-binding site (CBS) in each domain. In this study, we resolved two crystal structures of the Na+-bound form at high resolution (1.58 and 1.35 Å). This is the first report that Anx4 binds the Na+ ion in CBSs. Electron density maps, valence screening, and atomic absorbance spectrometry confirmed that Anx4 bound the Na+ ion. One structure (1.58 Å) bound the Na+ ion in CBS I, whereas another structure (1.35 Å) bound the Na+ ion in CBS II and CBS III. We compared the two Na+-bound forms by superimposing their Cα traces. The Cα atoms of CBS III largely moved by coordination of the Na+ ion. In the Cα atoms of CBS I, however, little change resulted from Na+-coordination. Only the side chain of Glu71 was moved by Na+-coordination in CBS I. These results indicate that Anx4 also binds not only Ca2+ but also Na+ ion in the CBS.

著者

• Butsushita Kohei

  Biological Science Course, Graduate School of Science and Engineering, Aoyama Gakuin University

• Fukuoka Shin-Ichi

  Biological Science Course, Graduate School of Science and Engineering, Aoyama Gakuin University

• Ida Koh

  Department of Biosciences, Kitasato University

• ARII Yasuhiro

  Biological Science Course, Graduate School of Science and Engineering, Aoyama Gakuin University

関連論文

• Crystal Structures of Sodium-Bound Annexin A4
• Production of a Recombinant Full-Length Prion Protein in a Soluble Form without Refolding or Detergents
• Crystal Structures of Sodium-Bound Annexin A4

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