Studies on Neurosteroids XXVI. Fluoxetine-Evoked Changes in Rat Brain and Serum Levels of Neuroactive Androgen, 5α-Androstane-3α,17β-diol
スポンサーリンク
概要
- 論文の詳細を見る
It is well known that fluoxetine (Fluox), a selective serotonin reuptake inhibitor, increases the brain content of allopregnanolone (AP), a potent neuroactive steroid that positively modulates the action of γ-aminobutyric acid (GABA) at the GABA type A receptors, but the influence of Fluox on the brain and serum levels of a neuroactive androgen, 5α-androstane-3α,17β-diol (3α,5α-Adiol), is poorly understood. In this study, we examined the Fluox-evoked changes in the 3α,5α-Adiol levels and compared the level changes of 3α,5α-Adiol with those of AP. The brain and serum 3α,5α-Adiol and AP levels were determined using previously developed LC-MS/MS. The ratio of the brain 3α,5α-Adiol to the serum 3α,5α-Adiol concentrations (B/S value) was significantly elevated in the rats intraperitoneally administered Fluox (10 mg/kg). Although the magnitude of the Fluox-evoked level change in 3α,5α-Adiol was much lower than that in AP, this study demonstrated that the 3α,5α-Adiol content is also influenced by Fluox. The most probable cause for the increase in the B/S value by the Fluox treatment is the activation of the 3α-hydroxysteroid dehydrogenase enzyme followed by the promotion of the de novo biosynthesis of 3α,5α-Adiol in the brain.
- 公益社団法人 日本薬学会の論文
著者
-
NAGURA Yukiko
Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa Uni
-
SHIMADA Kazutake
Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa Uni
-
Shimada Kazutake
Division Of Pharmaceutical Sciences Graduate School Of Natural Science And Technology Kanazawa Unive
-
Higashi Tatsuya
School of Pharmaceutical Sciences, University of Shizuoka
-
Toyooka Toshimasa
School of Pharmaceutical Sciences, University of Shizuoka
-
Shimada Kazutake
Kanazawa University
-
Higashi Tatsuya
School Of Pharmaceutical Sciences University Of Shizuoka
関連論文
- Studies on Neurosteroids XXV. Influence of a 5α-Reductase Inhibitor, Finasteride, on Rat Brain Neurosteroid Levels and Metabolism(Analytical Biochemistry)
- Studies on Neurosteroids XXI : An Improved Liquid Chromatography-Tandem Mass Spectrometric Method for Determination of 5α-Androstane-3α,17β-diol in Rat Brains
- Contribution of glucuronic acid and sulfonic acid moieties during photocatalytic degradation of estrogen conjugates
- Studies on Neurosteroids XXVI. Fluoxetine-Evoked Changes in Rat Brain and Serum Levels of Neuroactive Androgen, 5α-Androstane-3α,17β-diol
- Alternative Procedure for Charged Derivatization to Enhance Detection Responses of Steroids in Electrospray Ionization-MS
- Simultaneous determination of salivary testosterone and dehydroepiandrosterone using LC-MS/MS: Method development and evaluation of applicability for diagnosis and medication for late-onset hypogonadism
- Liquid chromatography-tandem mass spectrometric method for determination of salivary 17α-hydroxyprogesterone : A noninvasive tool for evaluating efficacy of hormone replacement therapy in congenital adrenal hyperplasia
- Determination of digoxin in human serum using stable isotope dilution liquid chromatography/electrospray ionization-tandem mass spectrometry
- Advances in determination of vitamin D related compounds in biological samples using liquid chromatography-mass spectrometry : A review
- Determination of salivary dehydroepiandrosterone using liquid chromatography-tandem mass spectrometry combined with charged derivatization
- Studies on neurosteroids XIX Development and validation of liquid chromatography-tandem mass spectrometric method for determination of 5α-reduced pregnane-type neurosteroids in rat brain and serum
- 2-Hydrazino-1-methylpyridine : a highly sensitive derivatization reagent for oxosteroids in liquid chromatography-electrospray ionization-mass spectrometry
- Application of Monolithic Silica Column for HPLC Separation of Estrogens