Somatic chromosome differentiation in three species of the Byblis liniflora complex (Byblidaceae)
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概要
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We conducted chromosomal characterizations of three species in the <I>Byblis liniflora</I> complex using sequential fluorescent staining with chromomycin A<SUB>3</SUB> (CMA) and 4',6-diamidino-2-phenylindole (DAPI), and fluorescence <I>in situ</I> hybridization method. <I>Byblis filifolia</I> and <I>B. rorida</I> had the diploidal chromosome number of 2<I>n</I>=16, while <I>B. liniflora</I> had the tetraploidal chromosome number of 2<I>n</I>=32. All chromosomes of three species were median-centromeric. The primary constrictions, which contain centromeric regions, showed slightly CMA-positive stainability. Two CMA-positive DAPI-negative segments were observed on two chromosomes of the diploids <I>B. rorida</I> and <I>B. filifolia</I>, and the tetraploid <I>B. liniflora</I>. The 45S rDNA signals were detected on two chromosomes of the diploid and the tetraploid species, respectively. The positions of the 45S rDNA were corresponded to CMA-positive DAPI-negative segments.
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国際染色体植物学会 | 論文
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