The forward and backward stepping processes of kinesin are gated by ATP binding

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概要

• 論文の詳細を見る

• The kinesin motor converts the chemical energy from ATP turnover into mechanical work, which produces successive 8-nm steps in the forward and backward direction along a microtubule. A key problem for kinesin mechanochemistry is explaining how ATP turnover is coordinated with mechanical work. We investigated this by measuring the ATP dependent properties of kinesin forward and backward steps using optical trapping nanometry. The results showed that the rate for both forward and backward steps are ATP-dependent, indicating that ATP binding to kinesin triggers both forward and backward steps. This suggests that ATP turnover in kinesin is not rigidly coupled to total mechanical work at high load.

• 日本生物物理学会の論文

著者

• Yanagida Toshio

  Laboratories For Nanobiology Graduate School Of Frontier Bioscience Osaka Univ.

• Yanagida Toshio

  Laboratories for Nanobiology, Graduate School of Frontier Biosciences, Osaka University

• Taniguchi Yuichi

  Department of Chemistry and Chemical Biology, Harvard University

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• The forward and backward stepping processes of kinesin are gated by ATP binding

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