Existence of Ionotropic Glutamate Receptor Subtypes in Cultured Rat Retinal Ganglion Cells Obtained by the Magnetic Cell Sorter Method and Inhibitory Effects of 20-Hydroxyecdysone, a Neurosteroid, on the Glutamate Response
スポンサーリンク
概要
- 論文の詳細を見る
Glutamate and neurosteroids are known to exist in retinal ganglion cells (RGC). Therefore, patch clamp studies using the whole-cell recording method were performed to determine whether or not ionotropic glutamate receptor subtypes, i.e., N-methyl-<sc>D</sc>-aspartate (NMDA), α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) and kainate receptors, were present on RGC obtained by the magnetic cell sorter (MACS) method and cultures. In addition, the effects of 20-hydroxyecdysone (20-HE), a neurosteroid, on inward currents induced by NMDA, AMPA and kainate were examined at a holding potential of −60 mV. The current-voltage relationship for NMDA in the presence of glycine and Mg2+-free, as well as those for AMPA and kainate were linear, with a reversal potential of around 0 mV. NMDA-induced currents were blocked by MK-801, while both AMPA- and kainate-induced currents were blocked by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX). Application of 20-HE in the bath resulted in significant inhibitions on NMDA-, AMPA- and kainate-induced currents. Thus, NMDA, AMPA and kainate receptors were confirmed to exist on MACS-separated cultured RGC. Moreover, 20-HE inhibited NMDA receptor-mediated currents most prominently and AMPA- and kainate-mediated currents moderately, suggesting that neurosteroids may be playing a role in modulating glutamate-mediated transmission in RGC, and 20-HE might be useful for preventing glutamate neurotoxicity.
- 社団法人 日本薬理学会の論文
- 2002-05-01
著者
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SASA Masashi
Department of Molecular and Pharmacological Neuroscience, Graduate School of Biomedical Sciences, Hi
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Sasa Masashi
広島大学 耳鼻咽喉科
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MISHIMA Hiromu
Department of Ophthalmology and Visual Science, Graduate School of Biomedical Sciences, Hiroshima Un
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ISHIHARA Kumatoshi
Department of Pharmacotherapy, Hiroshima University School of Medicine
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MUKAI Satoshi
Department of Ophthalmology Hiroshima University School of Medicine
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SHOGE Keisuke
Department of Ophthalmology Hiroshima University School of Medicine
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SHINYA Makoto
Department of Ophthalmology Hiroshima University School of Medicine
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Sasa M
Hiroshima Univ. School Of Medicine Hiroshima Jpn
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Mukai Satoshi
Department Of Ophthalmology And Visual Sciences Hiroshima University Graduate School Of Biomedical S
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Mishima H
Department Of Ophthalmology Hiroshima University Faculty Of Medicine
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Mishima Hiromu
Department Of Ophthalmology And Visual Science Division Of Frontier Medical Science Graduate School
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Ishihara Kumatoshi
Department Of Pharmacology Faculty Of Pharmaceutical Sciences Kyoto University
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Ishihara Kumatoshi
Department Of Pharmacotherapy Graduate School Of Medical Sciences Hiroshima University
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Ishihara Kumatoshi
Department of Neurology and Neurosurgery, The Montreal Neurological Institute and Hospital
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