Construction of a BAC Library for Chinese Amphioxus Branchiostoma belcheri and Identification of Clones Containing Amphi-Pax Genes
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概要
- 論文の詳細を見る
Amphioxus is a crucial organism for the study of vertebrate evolution. Although a genomic BAC library of Branchiostoma floridae has been constructed, we report here another BAC library construction of its distant relative species Branchiostoma belcheri. The amphioxus BAC library established in present study consists of 45,312 clones arrayed in one hundred and eighteen 384-well plates. The average insert fragment size was 120 kb estimated by Pulsed Field Gel Electrophoresis (PFGE) analysis of 318 randomly selected clones. The representation of the library is about 12 equivalent to the genome, allowing a 99.9995% probability of recovering any specific sequence of interest. We further screened the library with 4 single copied Amphi-Pax genes and identified total of 26 positive clones with average of 6.5 clones for each gene. The result indicates this library is well suited for many applications and should also serve as a useful complemental resource for the scientific community.
- 日本遺伝学会の論文
著者
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WANG Wei
Key Laboratory of Pharmacokinetics and Drug Metabolism, China Pharmaceutical University
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Xu Huai-Liang
Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, The Chinese Academ
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Lin Lu-Ping
Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, School of L
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Su Bing
Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, The Chinese Academ
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Wang Yi-Quan
Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, School of L
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Wang Wei
Key Lab. Of Ministry Of Education For Coastal And Wetland Ecosystems School Of Life Sciences Xiamen
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Wang Yi-quan
Key Laboratory Of Ministry Of Education For Coastal And Wetland Ecosystems School Of Life Sciences X
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Wang Wei
Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University
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Xu Huai-Liang
Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, The Chinese Academy of Sciences
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