培養ヒト2倍体細胞における突然変異の研究-1-細胞の生存に対する突然変異の物質及び選択物質の作用〔英文〕

概要

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Using human diploid cells derived from a 5-month embryonic lung studies were made on optimal culture conditions for colony formation (plating efficiency) and the effects of some chemical mutagens and a selective agent on the plating efficiency.Among the various media tested, Ham's medium F12 supplemented with 10% calf serum gave the highest plating efficiency, followed by Eagle's basal medium, Eagle's minimum essential medium, each supplemented with 10% calf serum, Puck's medium N16CF and Puck's medium F16 with 10% calf serum.The effects of various concentrations of ethyl methanesulfonate (EMS), N-methyl- N'-nitro-N-nitrosoguanidine (MNNG) and 8-azaguanine (8AG) on the plating efficiency of cells were tested and D0 values were calculated from the concentration-survival curves. The D0 value for 14 day-treatment with EMS was 9.6×10-4M. The D0 values for 2 hour- and 14 day-treatments with MNNG were 1.2×10-5M and 1.1×10-5M, respectively. The D0 value for 8AG was 0.73μg/ml (4.8×10-6M).
Genetics Society of Japanの論文