Genetic relationships of Pinanga coronata accessions by RAPD Markers
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概要
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Genetic relationships among 16 accessions of <I>Pinanga coronata</I> (Blume <I>ex</I> Mart.) Blume from different sources both wild and cultivated individuals from botanical gardens (Indonesian Botanic Gardens and Montgomery Botanical Center) were studied using Random Amplified Polymorphic DNA (RAPD) markers. Thirty of fourty primers were reproducible among the 16 accessions of <I>P. coronata</I>. They revealed a total 243 bands, of which 238 were polymorphics. The genetic similarity according to Jaccard's coefficients among accessions ranged from 0.115 to 0.771, which was indicating that the accessions were genetically very diverse. Cluster analysis by UPGMA showed two clusters. Cluster A consisted of eight accessions from Sumatra (PC<SUB>1</SUB>) and Java (PC<SUB>2</SUB>, PC<SUB>4</SUB>, PC<SUB>6</SUB>, PC<SUB>7</SUB>, PC<SUB>8</SUB>, PC<SUB>9</SUB>, and PC<SUB>10</SUB>) in which genetic similarity varied between 0.50 to 0.63; and cluster B consisted of five accessions from Bali Isl. (PC<SUB>12</SUB>, PC<SUB>13</SUB>, and PC<SUB>14</SUB>), Sumbawa Isl. (PC<SUB>15</SUB>) and Timor Isl. (PC<SUB>16</SUB>) in which genetic similarity varied between 0.56 and 0.77. Three accessions from Java (PC<SUB>3</SUB>, and PC<SUB>5</SUB>) and Bali Isl. (PC<SUB>11</SUB>) did not belong to any clusters. Based on this study, cluster separation was not only depend on altitudes, but also geographical distribution as well.
- 国際染色体植物学会の論文
国際染色体植物学会 | 論文
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