フタバガキ科植物リュウノウジュにおけるマイクロサテライト多型〔英文〕

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概要

• 論文の詳細を見る

• Di-nucleotide microsatellites were isolated from a genomic library of a tropical tree species, Dryobalanops lanceolata, in Sarawak, for the purpose of using them as hypervariable genetic markers to study the pollen-mediated gene flow. Among 1600 recombinant clones, in total 20 clones gave positive signals when hybridized with oligonucleotides with the three different repeat motifs, GT, CA and CT. Estimations of abundance of (GT)n/(CA)n and (GA)n/(CT) n di-nucleotide repeats in D. lanceolata genome revealed to be one in every 84 kb and 80 kb, respectively. Among six sequenced microsatellite loci, one was selected to synthesize PCR primers to amplify the microsatellite. PCR product size of the locus was variable among different individuals, which is attributed to the different number of di-nucleotide repeats. The same microsatellite genotype was detected in the trunk and canopy of a single large tree, indicating the utility of trunk tissue as the source of DNA for the population genetic study of tropical tree species, the canopy of which is usually difficult to approach.

• 日本遺伝学会の論文
• 1994-10-25

著者

• Terauchi Ryohei

  Laboratory Of Plant Systematics Department Of Botany Kyoto University

関連論文

• Phylogenetic relationship among the section Stenophora in the genus Dioscorea based on the analysis of nucleotide sequence variation in the phosphoglucose isomerase(Pgi)locus
• フタバガキ科植物リュウノウジュにおけるマイクロサテライト多型〔英文〕

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