Amyloid Fibril Formation of a Simple Decapeptide in a Micellar Environment
スポンサーリンク
概要
- 論文の詳細を見る
A decapeptide Ala-Nle-Ala-Lys-Ala-Ala-Ala-Ala-Nle-Ala showed conformational transition from α-helix to β-sheet and further formed amyloid fibrils in a micellar environment in previous work with lysine-scanning decapeptides. In the present work we used norleucine-scanning decapeptides to evaluate amyloid formation under the same conditions. The decapeptides were synthesized, and their conformations were determined by circular dichroism (CD). Conformational transition from α-helix to β-structure was noted in nearly every decapeptide. However, only the decapeptide Ala-Ala-Ala-Lys-Ala-Ala-Ala-Nle-Ala-Ala (8X4K), which has one norleucine (Nle) and one lysine (Lys) in an alanine(Ala)-based decapeptide, formed the β-structure immediately and not via α-helices. A series of norleucine-scanning decapeptides were examined for amyloid-forming capacity, by using a fluorescence dye, Thioflavin T. An increase in fluorescence was also a clear indication of the high capacity of 8X4K to form amyloids. The amyloid aggregates were observed by transmission electron microscopy (TEM) and identified as parallel β-sheet structures by fourier transform infrared spectroscopy (FTIR).
- 日本油化学会の論文
著者
-
Akisada H
Department Of Biological Substances And Life Science Faculty Of Engineering Kyushu Kyoritsu Universi
-
Akisada Hideo
Department Of Biological Substances And Life Science Faculty Of Engineering Kyushu Kyoritsu Universi
-
Kato Tamaki
Graduate School Of Life Science And Systems Engineering Kyushu Institute Of Technology
-
KUWAHARA Junko
Department of Biological Substances and Life Science, Faculty of Engineering, Kyushu Kyoritsu Univer
-
Kuwahara Junko
Department Of Life Environment And Material Science Faculty Of Engineering Fukuoka Institute Of Tech
-
Nishino Norikazu
Graduate School Of Life Science And Systems Engineering Kyushu Instetute Of Technology
-
WAKAYAMA Noboru
Faculty of Engineering, Kyushu Institute of Technology
-
KUWAHARA Junko
Department of Biological Substances and Life Science, Faculty of Engineering, Kyushu Kyoritsu University
-
AKISADA Hideo
Department of Biological Substances and Life Science, Faculty of Engineering, Kyushu Kyoritsu University
関連論文
- Trisbipyridine Metal Ion's Nest in Three α-Helix Bundle Structure
- Alamethicin Oligomers Tethered on Cyclic Pseudopeptides
- Synthesis and Properties of Cyclic Pseudopeptides Containing Dipeptide Isosteres
- Effects of Shear Stress on the Self-Assembly Process of Elastomeric Polypeptides
- Synthesis and Characterization of Peptide-Surfactants Using Elastin Repeating Unit
- Fluorescence Energy Transfer to Tailed Acridine Oranges from Pyrene Excimer in Four α-Helix Bundle 53-peptide
- Synthesis of Chlamydocin and HC-toxin Hydroxamic Acid Analogs Containing Acyclic Imino Acids and Conformational Effect in Inhibition of Histone Deacetylases
- HC-toxin and Chlamydocin Analogs Bearing Hydroxamic Acid as Histone Deacetylase Inhibitors
- Synthesis and Properties of Cyclic Peptides Arranging a Pair of Porphyrin and Fullerene C_
- Aromatic Ring Shifting in Chlamydocin Framework for Specific Inhibition of Histone Deacetylase Paralogs
- Design and Synthesis of Histone Deacetylase Inhibitors Containing Hydroxy-Imino-Acids as the Capping Group
- 2P585 Toward Potent HDAC Inhibitor Design : Insights from Homology Modeling and Docking Simulation of HDAC and Its Inhibitors(55. Drug design and delivery,Poster Session,Abstract,Meeting Program of EABS &BSJ 2006)
- Design and Synthesis of Histone Deacetylase Inhibitors Containing Trifluoromethylketone Moiety as the Functional Group
- Facile Synthesis of Modified Cyclic Imino Acids with Methyl and Hydroxyl Groups
- Advanced Technology for the Production of Monoclonal Antibodies Based on In Vitro Immunization
- Effects of Hydrophobic and Electrostatic Interactions on Change in Decapeptide Conformation in Micelle and Liposome Solution
- Rapid Generation of Monoclonal Antibodies against the Epitope Sequences of Human Insulin Using an In Vitro Immunization Method
- Aniline-Hydroxylation Activity of a Flavin-linked βαβα-Type Polypeptide Packing an Iron Porphyrin
- Complexation of 4,4'-Dipyridyl Derivatives Changed the Orientations of Metalloporphyrins Linked to the Cyclic Peptide Gramicidin S
- An Attempt to Build Electron Transfer System in Dendritic Poly(L-Lysine)
- Analysis of Epitope Sequences Based on B Cell Targeting Technique
- CD Investigation of Porphyrin-Porphyrin Interaction with Links to Acyclic β-Sheet Peptide Self-Assembled in an Aqueous Media
- Synthesis of [Hexafluorovalyl^]gramicidin S
- Self-Assembling of Porphyrin-Linked Amphiphilic β-Sheet Peptides
- Lipidation of the Collagen Model Peptide to Make Peptide-Surfactant
- Two Types of Cation Binding Sites on Elastomeric Polypeptides : Effects on Self-Assembly Process and Elastomeric Functions
- Self-assembling Cyclic Peptides for Peptide Nanotube Formation
- The NMR Spectroscopic Evaluation of Immobility of a Crowd of Porphyrin Rings Combined with Dendritic Poly(L-lysine)s
- The Conformation of de Novo Designed Amphiphilic Peptides with Six or Nine _L-2-(2,2,2-Trifluoroethyl) glycines as the Hydrophobic Amino Acid
- Fluorescence Energy Transfer in Dendritic Poly(L-lysine)s Combining Thirty-two Free Base-and Zinc(II)-porphyrins in Scramble Fashion
- De Novo Design of Hemoprotein Model with Peroxidase Activity toward Lipophilic Peroxide
- Synthesis of Cyclic Tetrapeptides Containing Non-natural Imino Acids
- High Throughput Screening System for Histone Methyltransferase Inhibitors
- Host-Guest Study of Collagen Model Peptide with Pipecolic Acid
- Helical Propensity of Pipecolic Acid in Collagen Model Peptides
- Synthesis and Characterization of Collagen Model Peptides with A Pyrene Group at the Amino-Terminal
- Facile Preparation of Hydroxyproline Derivatives and Separation of Stereoisomers
- Synthesis and Characterization of Antimicrobial Peptide Surfactants with Dendritic Lysine Groups
- Bicyclic Tetrapeptides with Disulfide as Histone Deacetylase Inhibitors
- Evaluation of Functional Groups as HDAC Inhibitors on Monopeptide Derivatives
- Self-assembling Cyclic Hexapeptides for Peptide Nanotube Formation
- Efficient Production of Monoclonal Antibodies Specific to Aimed Epitope Sequences by a B Cell Targeting Technique
- Cyclization Test and Optimization of Cyclization Condition for Cyclic Tetrapeptides with Various Configurations
- Cyclic Tetrapeptides containing N-Isopropylglycine as Histone Deacetylase Inhibitors
- Synthesis and Activity of Cyl-1 Analogs Having Hydroxamic Acid at Side Chain
- Histone Deacetylase Inhibitors Based on Trapoxin B
- Conformational Analysis of Non-Natural LDLD-Type Cyl-1 Analog with High Activity
- Amyloid Fibril Formation of a Simple Decapeptide in a Micellar Environment
- Extraction of Collagen from Fishery Waste and Effects of Cross-Linking Structures
- Simultaneous Detection on Antimicrobial Activities of Dendritic-L-Lys-Polystyrene Beads and Extracellular Enzyme Activities in Culture Media
- Sequence Dependence in Cyclization Reaction for Cyclic Tetrapeptides Different in Configurations
- Self-assembly of Dialkyl-type Surfactants Bound to Amino Acids and Peptides in Aqueous Media