Capacitation and Fertilizing Ability of BALB/c Mouse Spermatozoa Collected from Different Regions of the Cauda Epididymis

スポンサーリンク

概要

• 論文の詳細を見る

• To investigate whether the fertilizing ability is changed during transit of spermatozoa in the cauda epididymis in BALB/c mouse which is known as a low in vitro fertilization (IVF) rate, we examined the capacitation rates of spermatozoa collected from 3 regions of the cauda epididymis by chlortetracycline (CTC) fluorescence assay. In addition, their fertilizing ability was examined by IVF with cumulus-intact and cumulus-free mouse eggs. Spermatozoa immediately collected from the cauda epididymis showed no significant differences in capacitation rates (the proportion of spermatozoa having a fluorescence-free dark band in the postacrosomal region; B pattern) estimated by CTC test among regions. Nevertheless, the capacitation rates in the proximal region were significantly (P<0.05) higher than that in distal region at 60 min of incubation and the middle region at 120 min of incubation. Non-preincubated spermatozoa regardless of the collection regions in the cauda epididymis showed signs of low fertilizing ability in cumulus-intact (9-10%) and cumulus-free (0-4%) mouse eggs at 6 h after insemination. After 2 h sperm preincubation, fertilization rates of spermatozoa collected from the proximal and distal regions were only slightly increased (26-44%) but that of middle region was greatly increased in both cumulus-intact (68%) and cumulus-free (61%) mouse eggs. These results suggest that the fertilizing ability of BALB/c mouse spermatozoa is changed during transit of the cauda epididymis, and the spermatozoa in the middle region of the cauda epididymis could be utilized efficiently for IVF in the BALB/c mouse strain.

• 日本繁殖生物学会の論文

著者

• Seng Seyha

  Research Center For Protozoan Molecular Immunology Obihiro University Of Agriculture And Veterinary

• Choi Young-ho

  Research Center For Protozoan Molecular Immunology Obihiro University Of Agriculture And Veterinary

• TOYODA Yutaka

  Research Center for Molecular Protozoology, Obihiro University of Agriculture and Veterinary Medicin

• BOONCHIT Suthisak

  Research Center for Protozoan Molecular Immunology, Obihiro University of Agriculture and Veterinary

• Boonchit Suthisak

  Research Center For Protozoan Molecular Immunology Obihiro University Of Agriculture And Veterinary

関連論文

• Identification of Carbohydrates on Eimeria stiedai Sporozoites and Their Role in Invasion of Cultured Cells in vitro.
• BALB/cおよびICRマウス精巣上体精子の受精能獲得および受精能力の比較:卵丘存在および除去マウス卵子の体外受精による解析
• Effect of Taurine on In Vitro Fertilization and Embryo Development of BALB/c Mouse Strain
• Capacitation and Fertilizing Ability of BALB/c Mouse Spermatozoa Collected from Different Regions of the Cauda Epididymis
• Establishment of Embryonic Stem (ES) Cell-Like Cell Lines Derived from Bovine Blastocysts Obtained by In Vitro Culture of Oocytes Matured and Fertilized In Vitro
• Capacitation and Fertilizing Ability of BALB/c Mouse Spermatozoa Collected from Different Regions of the Cauda Epididymis
• Exposure of C57BL/6J Male Mice to an Electric Field Improves Copulation Rates with Superovulated Females
• Effect of Taurine on In Vitro Fertilization and Embryo Development of BALB/c Mouse Strain
• C57BL/6J雄マウスの過剰排卵処理メスとの交尾率におよぼす電界曝露の効果

スポンサーリンク