Effects of Ghrelin on In Vitro Development of Porcine In Vitro Fertilized and Parthenogenetic Embryos
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概要
- 論文の詳細を見る
The objective of the present study was to investigate the effect of addition of ghrelin to in vitro culture medium on preimplantation development of porcine in vitro fertilized and parthenogenetic embryos. In Experiment 1, we sought to compare the in vitro developmental competence of IVF and parthenogenetic embryos. No significant (P<0.05) differences were detected for cleavage rate or blastocyst rate between the in vitro fertilization- and parthenogenetic activation-derived embryos. In Experiment 2, parthenogenetic embryos were cultured in Porcine Zygote Medium-3 containing various concentrations of ghrelin. The blastocyst rate was remakably (P<0.05) increased when 5 ng/ml (PA-5) and 500 ng/ml (PA-500) of ghrelin was added to in vitro culture medium compared with the other groups. Total cell number per blastocyst was slightly promoted in the ghrelin treatment groups compared with the controls. However, the ratio of ICM/total cell number was significantly reduced in the PA-50 group compared with the controls (P<0.05). In Experiment 3, we cultured in vitro fertilized embryos in Porcine Zygote Medium-3 supplemented with ghrelin at different dosages. The rate of blasotcyst formation was markedly (P<0.05) elevated when 500 ng/ml ghrelin was added to culture medium (IVF-500) compared with the controls. Increased total cell numbers (P<0.05) were observed when in vitro fertilized embryos were cultured in IVF-50 and IVF-500 compared with the controls. However, the ratio of ICM/total cell number was decreased in the ghrelin treatment groups compared with the controls (P<0.05). Taken together, the results suggest that ghrelin can enhance blastocyst formation of porcine in vitro fertilized and parthenogenetic embryos while exerting a negative effect on the structural integrity of the blastocysts.
- 日本繁殖生物学会の論文
著者
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Zhang Kun
State Key Laboratory For Agrobiotechnology College Of Biological Science China Agricultural Universi
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Li Ning
State Key Laboratory For Agrobiotechnology College Of Biological Science China Agricultural Universi
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WEI Heng-xi
State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural Univer
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Zhang Yun-Hai
Danish Institute of Agricultural Sciences
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WANG Shao-Hua
State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural Univer
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LI Yan
JiPulin Biotech Ltd.
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DAI Yun-Ping
State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural Univer
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Wei Heng-xi
State Key Laboratory For Agrobiotechnology College Of Biological Science China Agricultural Universi
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Zhang Kun
State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University
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DAI Yun-Ping
State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University
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WANG Shao-Hua
State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University
関連論文
- Stage-dependent Effect of Leptin on Development of Porcine Embryos Derived from Parthenogenetic Activation and Transgenic Somatic Cell Nuclear Transfer
- Effects of Ghrelin on In Vitro Development of Porcine In Vitro Fertilized and Parthenogenetic Embryos
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