Identification of a Novel Yolk Protein in the Hermatypic Coral Galaxea fascicularis
スポンサーリンク
概要
- 論文の詳細を見る
The reef-building (or hermatypic) coral Galaxea fascicularis (Anthozoa, Hexacorallia, Scleractinia) has an annual reproductive cycle. Females of G. fascicularis release packages (or `bundles) of eggs for external fertilization, whereas male individuals form bundles consisting of sperm and infertile `pseudo-eggs that are thought to confer buoyancy to the male bundle. In the egg of G. fascicularis, four proteins (GfEP-1 to 4) were found to be stored in high abundance, and three of them (GfEP-1, 2 and 3) are generated by processing of a vitellogenin (Vg)-like precursor. In the present study, a cDNA encoding GfEP-4 was cloned and its sequence determined (GenBank/EMBL/DDBJ accession no. AB259859). The amino acid sequence of this protein does not exhibit similarity to known proteins, including Vgs or other yolk proteins found in some invertebrates. The expression of GfEP-4 mRNA was observed in females, and also in the majority of males examined, although expression levels were lower than in females. The GfEP-4 protein was detected in pseudo-eggs, where its concentration was 20-100 times lower than in eggs. In contrast, GfEP-1, 2 and 3 were not detected in pseudo-eggs. A protein (28 kDa) which cross-reacted with anti-GfEP-4 antibodies was detected in eggs of the coral Montipora digitata, suggesting the possibility that homologs of this protein are present in the eggs of other scleractinian corals.
著者
-
Hayakawa Hideki
Division of Molecular Marine Biology, Ocean Research Institute, The University of Tokyo
-
Andoh Tadashi
Coastal Fisheries and Aquaculture Division, Hokkaido National Fisheries Research Institute, Fisherie
-
Watanabe Toshiki
Division of Molecular Marine Biology, Ocean Research Institute, The University of Tokyo
-
Watanabe Toshiki
Department Of Marine Bioscience Ocean Research Institute The University Of Tokyo
-
Hayakawa Hideki
Division Of Marine Biosciences Ocean Research Institute The University Of Tokyo
-
Watanabe Toshiki
Division Of Marine Bioscience Ocean Research Institute The University Of Tokyo
-
Andoh Tadashi
Coastal Fisheries and Aquaculture Division, Hokkaido National Fisheries Research Institute, Fisheries Research Agency
関連論文
- Identification of a Novel Yolk Protein in the Hermatypic Coral Galaxea fascicularis
- Breeding experiments of hermatypic coral Galaxea fascicularis : partial reproductive isolation between colonies of different nematocyst types and enhancement of fertilization success by presence of parental colonies
- Polymorphism in Nucleotide Sequence of Mitochondrial Intergenic Region in Scleractinian Coral (Galaxea fascicularis)
- 微量の硫黄を添加したFe-20Cr-4Al合金の高温酸化における硫黄の挙動
- Cloning and expression of a cDNA encoding an insoluble matrix protein in the gastroliths of a crayfish, Procambarus clarkii
- 微量の活性元素を添加したNi-20Cr-3Si合金のN_2-H_2O-HBr雰囲気における高温腐食
- Genetic and morphological differentiation in the hermatypic coral Galaxea fascicularis in Okinawa, Japan
- ANALYSIS OF A PRECURSOR STRUCTURE AND EXPRESSION OF EGG PROTEINS IN A REEF-BUILDING CORAL(Genetics,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- MOLECULAR ANALYSIS OF EGG PROTEINS IN REEF-BUILDING CORALS(Developmental Biology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- Identification of a novel cuticular protein in the kuruma prawn Penaeus japonicus