無機化学物質のオオミジンコ急性遊泳試験と培地におけるキレート剤の影響

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Abstract：Various metal salts exist in environment and they come from not only natural source but also artificial source. We expect that the metal salts also affect organisms (for example the metal ion) since metal chloride is one of the forms which are stabilized as a metal salt. Therefore, the toxic strength of metal chloride is investigated by Daphnia magna immobilization assay. Lithium chloride (LiCl), sodium chloride (NaCl), potassium chloride (KCl) rubidium chloride (RbCl) , and Cesium chloride (CsCl) are used as test reagent. D. magna immobilization test was carried out based on OECD Guidelines for the Testing of Chemicals (TG202). 20 neonates within 24 hours old were placed into M4 medium (exposure and control) under constant temperature (20±2℃). The neonates were observed at 24 and 48 hours after exposure and the number of affected and survived neonates were recorded. The EC50 in M4 medium was 61.6 mg LiCl / L, 5100 mg NaCl / L, 769.7 mg KCl / L, 77.8 mg RbCl / L, and 51.5 mg CsCl / L. The EC50 in M4 medium without EDTA was 21.7 mg LiCl / L, 2254 mg NaCl / L, 674 mg KCl / L, 64.5 mg RbCl / L, and 35.4 mg CsCl / L. These results correlate with chelate formation constant (logKf). キーワード：オオミジンコ, 無機化学物質, 急性遊泳阻害試験, 塩化物, EDTA, M4培地
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