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概要
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Following rapid progress in human genetics research, the importance of construction of bio-resource banking system grows in the world. Whole genome association studies can identify gene polymorphisms in human DNA that cause particular diseases or are responsible for effects of medicines. However, identification of these genetic factors need many clinical and control cases with the DNA and serum in blood sample. Bio-resource banking system has already established in humans and allows us to use the large population samples. Dogs share many diseases with humans and bio-resource banking system is also useful for genetics research in dogs. The objective of this study is to develop canine bio-resource banking system in Japan. For extraction of DNA from blood samples, we compared the efficacy of DNA extraction kit between Genomix 2.4 blood and QIAamp DNA Blood Midi Kit. Eleven referral veterinary animal hospitals agreed with canine bio-resource banking project in Japan. Following allowance of the use of blood samples and patient data from the owners, the veterinarian will send samples and patient data to our laboratory in Azabu University. Genomix 2.4 blood was selected for extraction of DNA from blood samples because Genomix 2.4 blood is superior to QIAamp DNA Blood Midi Kit in that the amount of DNA extraction from 2 ml of the blood sample. The developed canine bio-resource banking system would be a key source for genetics research in dogs. Progress of the research would lead to prevention and therapy of the genetic diseases in dogs.
- 麻布大学の論文
- 2009-03-31
麻布大学 | 論文
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