ウレアーゼ陽性高温性カンピロバクター（UPTC）のウレアーゼ遺伝子オペロンの分子解析

概要

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When cloning, sequencing and characterization of the genetic organization of urease genes within urease-positive thermophilic Campylobacter (UPTC) were carried out, an approximate 5.1 kilo base pair region encoding a urease gene operon was identified with recombinant plasmid DNAs from a genomic DNA library of a Japanese isolate (CF89-12). Six closely spaced and putative open reading frames (ORFs) for ureA, ureB, ureE, ureF, ureG and ureH were detected. ATG codons initiated each ORF of the UPTC urease operon except for ureB and ureH, which commenced with the most probable TTG codon. Overlaps were detected between ureA and ureB and also between ureB and ureE. Probable ribosome-binding sites and a putative p independent transcriptional termination region were identified. Two putative promoter structures, consisting of consensus sequences at the -35 and -10 like regions were also identified. Construction of a neighbor-joining tree based on the nucleotide sequence data of urease genes indicated that UPTC formed a cluster with some Helicobacter organisms separate from the other urease-producing bacteria, suggesting a commonly shared ancestry between UPTC and Helicobacter urease genes.
麻布大学の論文
2009-03-31