胎盤における一酸化窒素（NO）の産生とNO合成酵素（NOS）の発現
スポンサーリンク
概要
- 論文の詳細を見る
Nitric oxide (NO) production in the rat placenta(pars fetalis placentae and decidua basalis) was monitored and quantified by electron paramagnetic resonance (EPR) spectroscopy using an iron complex with N-(dithiocarboxy) sarcosine (Fe-DTCS) as a NO trapping reagent. The expression of nitric oxide synthase (NOS) isoforms was also examined by quantitative reverse transcriptase-mediated polymerase chain reaction (RT-PCR) analysis. The EPR spectrum of the placenta with Fe-DTCS trapping showed a triplet signal (g = 2.038) derived from an NO-Fe-DTCS complex. The heights of the NO-Fe-DTCS (a) and MnO (b) signals were simultaneously measured to calculate the ratio (a/b) of these signal heights used for the quantification of the NO production level. Although, the ratio of the signal heights of NO-Fe-DTCS and MnO did not vary significantly with gestational stage in the fetal placenta, the ratio was markedly decreased in the maternal placenta during the last few days of gestation. At the gestational stages examined, the level of NOS 2 (iNOS) mRNA expression was significantly higher than that of NOS 3 (eNOS) mRNA expression at any given stage in the fetal placenta, and the production pattern of NO was in good accordance with the expression pattern of NOS 2 in the maternal placenta. These results suggest that NOS 2 is the predominant producer of NO in the placenta and that NOS 2-generated NO plays significant roles in the maintenance of placental functions immediately before birth.
- 麻布大学の論文
- 2009-03-31
麻布大学 | 論文
- 研究科分 鶏の福祉ケージにおける設備配置と空間利用--砂浴び場および巣箱の配置が行動,健康状態および生産反応に及ぼす影響 (平成19年度麻布大学公的研究助成金事業研究成果報告)
- イノシシの行動制御技術開発のための嗅覚・聴覚刺激を用いた研究(I.大学院重点特別・研究科特別経費,平成18年度麻布大学公的研究助成金事業研究成果報告)
- 広域輸送される肥育素牛のストレスおよび動物福祉レベルの評価(I.大学院重点特別・研究科特別経費,平成18年度麻布大学公的研究助成金事業研究成果報告)
- 産卵鶏におけるバタリーケージの再評価 : 福祉ケージとの比較による多面的検討(I.大学院重点特別・研究科特別経費,平成18年度麻布大学公的研究助成金事業研究成果報告)
- 産卵鶏におけるケージ飼育の再評価 : 代替飼育法との比較による多面的検討