Arginine Inhibits Adsorption of Proteins on Polystyrene Surface
スポンサーリンク
概要
- 論文の詳細を見る
Nonspecific adsorption of protein on solid surfaces causes a reduction of concentration as well as enzyme inactivation during purification and storage. However, there are no versatile inhibitors of the adsorption between proteins and solid surfaces at low concentrations. Therefore, we examined additives for the prevention of protein adsorption on polystyrene particles (PS particles) as a commonly-used material for vessels such as disposable test tubes and microtubes. A protein solution was mixed with PS particles, and then adsorption of protein was monitored by the concentration and activity of protein in the supernatant after centrifugation. Five different proteins bound to PS particles through electrostatic, hydrophobic, and aromatic interactions, causing a decrease in protein concentration and loss of enzyme activity in the supernatant. Among the additives, including arginine hydrochloride (Arg), lysine hydrochloride, guanidine hydrochloride, NaCl, glycine, and glucose, Arg was most effective in preventing the binding of proteins to PS particles as well as activity loss. Moreover, even after the mixing of protein and PS particles, the addition of Arg caused desorption of the bound protein from PS particles. This study demonstrated a new function of Arg, which expands the potential for application of Arg to proteins.
- Public Library of Scienceの論文
Public Library of Science | 論文
- Soldier-Specific Modification of the Mandibular Motor Neurons in Termites
- Molecular Mapping of Movement-Associated Areas in the Avian Brain : A Motor Theory for Vocal Learning Origin
- Can Ethograms Be Automatically Generated Using Body Acceleration Data from Free-Ranging Birds?
- Cardiac Arrest during Gamete Release in Chum Salmon Regulated by the Parasympathetic Nerve System
- Effects of Active Conductance Distribution over Dendrites on the Synaptic Integration in an Identified Nonspiking Interneuron