Evaluation of BCMA as a New Color Reagent for the Choline and Spermine Tests

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bis[3-bis(4-Chlorophenyl)methyl-4-dimethylaminophenyl]amine (BCMA) was tested as a color reagent for the enzymatic choline and spermine tests for the identification of semen in stains. The sensitivity as a function of optical density with BCMA was about 10-fold higher than that with the 4-aminoantipyrine (4AA)-phenol system and about 5-fold higher than that with the 4AA-N-ethyl-N-(3-methylphenyl)-N'-acetylethylenediamine (EMAE) system. However, the high sensitivity of the BCMA did not appreciably contribute to sensitive detection of choline in the stains with the naked eye, because of the absorption maximum (755 nm) close to infrared wavelength of the color developed from BCMA. The choline test with BCMA observed with the naked eye showed that the method did not exceed that with the previous 4AA-EMAE system in sensitivity, specificity and stability. BCMA could not be used for the qualitative detection of spermine in stains because of its too low content in small seminal samples. BCMA seems more useful for quantitation of substrates by spectrophotometry than for discrimination of its color with the naked eye.rights:日本法医学会rights:本文データは日本法医学会の許諾のもと掲載しています。
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