Detection of Cannabinoids by Gas Chromatography/Mass Spectrometry (GC/MS)Part III. Negative Ion Chemical Ionization GC/MS of Cannabinoids in Human Materials

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Negative ion chemical ionization (CI) mass spectra of ⊿9-tetrahydrocannabinol, cannabidiol and cannabinol after derivatization with pentafluorobenzoyl (PFB) chloride and trifluoroacetic (TFA) anhydride were presented and compared with those in the positive CI and positive electron impact (EI) modes. The spectra in the negative CI mode showed strong molecular anions in the high mass number region especially after derivatization with PFB chloride. Using the molecular anions and a strong fragment ion, the quantitation of the three cannabinoids, which had been added to human urine and blood, was made by selected ion monitoring (SIM). The detection limit of the cannabinoids was less than 100 pg and 1.0 ng per injected volume for their PFB- and TFA-derivatives, respectively. The isolation procedure with Sephadex LH-20, which had been required for detection of cannabinoids in plasma in the positive EI mode, could be omitted because of the low backgrounds in SIM in the negative CI mode.rights:日本法医学会rights:本文データは日本法医学会の許諾のもと掲載しています。
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