Endogenous Synthesis of Corticosteroids in the Hippocampus
スポンサーリンク
概要
- 論文の詳細を見る
Background: Brain synthesis of steroids including sex-steroids is attracting much attention. The endogenous synthesis of corticosteroids in the hippocampus, however, has been doubted because of the inability to detect deoxycorticosterone (DOC) synthase, cytochrome P450(c21).Methodology/Principal Findings: The expression of P450(c21) was demonstrated using mRNA analysis and immmunogold electron microscopic analysis in the adult male rat hippocampus. DOC production from progesterone (PROG) was demonstrated by metabolism analysis of 3H-steroids. All the enzymes required for corticosteroid synthesis including P450(c21), P450(2D4), P450(11b1) and 3b-hydroxysteroid dehydrogenase (3b-HSD) were localized in the hippocampal principal neurons as shown via in situ hybridization and immunoelectron microscopic analysis. Accurate corticosteroid concentrations in rat hippocampus were determined by liquid chromatography-tandem mass spectrometry. In adrenalectomized rats, net hippocampus-synthesized corticosterone (CORT) and DOC were determined to 6.9 and 5.8 nM, respectively. Enhanced spinogenesis was observed in the hippocampus following application of low nanomolar (10 nM) doses of CORT for 1 h.Conclusions/Significance: These results imply the complete pathway of corticosteroid synthesis of 'pregnenolone RPROGRDOCRCORT' in the hippocampal neurons. Both P450(c21) and P450(2D4) can catalyze conversion of PROG to DOC. The low nanomolar level of CORT synthesized in hippocampal neurons may play a role in modulation of synaptic plasticity, in contrast to the stress effects by micromolar CORT from adrenal glands.
- Public Library of Scienceの論文
- 2011-00-00
Public Library of Science | 論文
- Soldier-Specific Modification of the Mandibular Motor Neurons in Termites
- Molecular Mapping of Movement-Associated Areas in the Avian Brain : A Motor Theory for Vocal Learning Origin
- Can Ethograms Be Automatically Generated Using Body Acceleration Data from Free-Ranging Birds?
- Cardiac Arrest during Gamete Release in Chum Salmon Regulated by the Parasympathetic Nerve System
- Effects of Active Conductance Distribution over Dendrites on the Synaptic Integration in an Identified Nonspiking Interneuron