Studies on chemical modification of papain by 5-chlorosulfonyl-2-oxobenzimidazole as biotin model compound
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5-chlorosulfonyl-2-oxobenzimidazole(１) was synthesized. On adding １ to the suspension of papain in acetonitrile containing formamide, １ was introduced into the papain in a yield of 7%, suggesting that １ modified papain chemically to give 2-oxobenzimidazolesulfonyl papain(OBI- papain). Also, it was found in-terestingly that papain activity of OBI-papain was maintained and that SH group in the active center in the large cleft of papain was free. Accordingly, It expects that OBI-papain might have other catalytic action except pa-pain activity. (accepted. Dec. 12, 2008)
- 大阪青山大学『大阪青山大学紀要』編集委員会の論文
- 2009-03-31
大阪青山大学『大阪青山大学紀要』編集委員会 | 論文
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