Photochemical identification of the binding region for (S)-semotiadil on sodium channels: Comparison with that for (R)-semotiadil on skeletal muscle calcium channel
スポンサーリンク
概要
- 論文の詳細を見る
To clarify the recognition site of (S)-semotiadil in the Na+ channels,we employed photoaffinity labeling with (S)-[3H]-D51-4700, an azidophenylderivative of (S)-semotiadil, to Na+ channel preparations from rat brains andporcine hearts, and the results were compared with those by (R)-enantiomer toskeletal muscle Ca2+ channel. Protease-digestion of the photolabeled Na+ channelsfollowed by quantitative immunoprecipitation assay using a site-directedantibody, revealed that 10- and 13- kDa fargments of brain and cardiac Na+channels, respectively, were located within the regions including IVS6. Theseregions are corresponding to the 8.3 kDa fragment photolabled by (R)-[3H]-D51-4700 in the skeletal muscle Ca2+ channel. Interestingly, however, thephotoincorporation of (S)-[3H]-D51-4700 into the Na+ channel was 2-3 timeshigher than that of (R)-isomer; and visa versa in the Ca2+ channels.
- The Japan Institute of Heterocyclic Chemistryの論文
- 2003-00-00
The Japan Institute of Heterocyclic Chemistry | 論文
- Catalytic Enantioselective Aziridination of Alkenes Using Chiral Dirhodium(II) Carboxylates
- Lewis Acid-Catalyzed Michael Addition Reactions of N-Boc-2-silyloxypyrroles to 3-Acryloyl-2-oxazolidinone
- Enantioselective Synthesis of 3-Arylindan-1-ones via Intramolecular C-H Insertion Reactions of α-Diazo-β-Ketoesters Catalyzed by Chiral Dirhodium(II) Carboxylates
- Dirhodium(II) Tetrakis[N-benzene-fused Phthaloyl-(S)-piperidinonate] as a Chiral Lewis Acid: Catalytic Enantioselective Aldol Reactions of Acetate-derived Silylketene Acetals and Aldehydes
- Direct and stereoselective synthesis of 2-azido-2-deoxy-β-D-mannosides using the phosphate method