Changes in granulation-formation induced by different fiber-diameters and fiber-spacing in scaffolds for tissue-regeneration : preliminary study
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概要
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目的:組織再生の足場は、直径が50～500nmである細胞外マトリックスの構造に可及的に類似することが望ましい。そこで、本実験では、直径0.7、0.9、3、7、20μmと繊維径の異なった5種類のポリグリコール酸繊維を用いて、5種類の不織布の足場を作製し、これらの足場が浸潤細胞及び肉芽組織形成に及ぼす影響を調べた。実験方法:6匹のラットの背部皮下組織に、それぞれ5つのポケットを作成し、5種類の足場を1つずつ別々のポケットに埋稙した。埋稙した足場とその周囲組織を、術後14日目に摘出し、病理組織学的に浸潤細胞及び肉芽組織形成の2つの観点から評価した。結果:繊維径0.9μmで繊維間隔10.8μmの足場と繊維径3μmで繊維間隔9.0μmの足場を用いた場合は、緻密な肉芽組織が足場の全層に渡り形成された。繊維径7μmで繊維間隔15.1μmの足場と繊維径20μmで繊維間隔31.6μmの足場を用いた場合は、疎な肉芽組織が繊維束間の空間に、やや疎な肉芽組織は、繊維束の周囲にのみ形成されていた。繊維径0.7μmで繊維間隔1.8μmの足場では、緻密な肉芽組織が足場の表面にのみ形成され、足場の内側へは細胞浸潤・肉芽組織形成は見られなかった。結論:繊維径3μm未満で繊維間隔が10μm付近の足場が、足場全層に渡り緻密な肉芽組織形成を引き起こし、組織再生の足場として最も適切であると考えられた。The fibers of the extracellular matrix (ECM) typically have diameters ranging from 50 to 500 nm. A scaffold should mimic the structure of the ECM as much as possible. As a result, 5 different fibers with diameters of 0.7, 0.9, 3, 7 and 20 μm were made into 5 kinds of scaffolds for tissue-regeneration. The scaffolds were made of Poly(glycolic acid) (PGA) nonwoven fabrics with fiber-spacing of 1.8 to 31.6 μm. This study investigated the influence of the 5 different scaffolds on infiltrating cells and granulation-formation. Six female Wistar rats underwent surgery to create 5 “pockets" in the subcutaneous tissue of the back, and one of the 5 types of PGA scaffolds was implanted into each “pocket". These implanted PGA scaffolds and surrounding tissues were excised en block on postoperative days 14, and then the infiltrating cells and granulation-formation were assessed microscopically. In the scaffolds with a fiber-diameters of 0.9 and 3 μm and fiber-spacing of 10.8 and 9.0 μm, respectively, dense granulation tissue was formed over the full-thickness of the scaffolds. However, in the scaffolds with fiber-diameters of 7 and 20 μm and fiber-spacing of 15.1 and 31.6 μm, respectively, a relatively sparse granulation tissue was formed only around the fiber bundles and sparse granulation tissue was observed to form in the spaces between the bundles. In the scaffolds with a fiber-diameter of 0.7 and fiber-spacing of 1.8 μm, dense granulation tissue only formed in the superficial and shallow layers of the scaffolds, and no cell-infiltration was found in the central region of the thickness. These results suggest that scaffolds with fiber-diameters smaller than 3 μm and fiber-spacing approximately 10 μm are suitable for artificial scaffolds made with PGA fibers to induce the regeneration of dense granulation tissue over the full-thickness of the scaffold.
- 2012-04-30
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