Studies on Myokinase in the Muscle of Fishes III : Catalytic Properties of Myokinase I and II from Carp Muscle
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概要
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Effect of bivalent metal ions on the activity of myokinase I and II from carp muscle was investigated. Co^++, Mn^++ and Mg^++ increased the activity of myokinase IIby 160, 118 and 84% respectively, but only Mg^++ increased the activity of myokinaseI slightly. EDTA inhibited the activity of myokinase II, but did not effect that ofmyokinase I. Myokinase II treated with EDTA was re-activated by dialyzing againstTris-Cl buffer (pH 7.6) containing MgCl_2 and MnCl_2. Mg^++ increased the thermalstability of myokinase II. Km values for ADP of myokinase I and II were 13.3 mMand 6.7 mM at 20°C respectively. Activated energies of myokinase I and II were6.75 and 5.61 Kcal per mole enzyme respectively.
- 1978-12-25