Further analysis on lidocaine-induced increase in intracellular Zn2+ concentration : Cytometric model study using FluoZin-3, 5-chloromethylfluorescein, and rat thymocytes
スポンサーリンク
概要
- 論文の詳細を見る
Lidocaine augmented FluoZin-3 fluorescence of rat thymocytes, suggesting the increase in intracellular Zn2+concentration. However, the mechanism for lidocaine-induced increase in the intensity of FluoZin-3fluorescence was not elucidated. In this study, in order to reveal the mechanism, the effects of lidocaine onFluoZin-3 and 5-chloromethylfluorescein (5-CMF) fluorescence were examined under various conditions.5-CMF fluorescence was used as a parameter for cellular content of nonprotein thiols. The lidocaine-inducedaugmentation of FluoZin-3 fluorescence was observed under external Ca2+- and/or Zn2+-free conditions,suggesting that lidocaine induced intracellular Zn2+ release. Lidocaine attenuated 5-CMF fluorescence,suggesting the decrease in cellular content of nonprotein thiols. Since some peptides consisting of SH-grouphave been proposed to store Zn2+, the decrease in nonprotein thiol content by
論文 | ランダム
- 中国経済第1次5ヵ年計画と水利事業
- 地方発 随想・国年事務と総務庁勧告--杉並区国年課長
- Monte Carlo Studies of the Ordering of the Three-Dimensional Isotropic Heisenberg Spin Glass in Magnetic Fields
- Synthesis of Epimeric 2-d_1-Dehydroepiandrosterones
- Stereospecific Hydrogen Transfer from C-4 to C-6 during Enzymatic Transformation of Cholesterol into Cholestenone