Fates of murine pluripotent stem cell-derived neural progenitors following transplantation into mouse cochleae.
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概要
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This study evaluated the tumorigenesis risk of induced pluripotent stem (iPS) cells after transplantation into the cochlea. One mouse embryonic stem (ES) cell line and three mouse iPS cell lines, one derived from adult mouse tail-tip fibroblasts (TTFs) and two from mouse embryonic fibroblasts (MEFs), were neurally induced by stromal cell-inducing activity. Before transplantation, the efficiency of neural induction and the proportion of residual undifferentiated cells were evaluated using immunocytochemistry, and no significant differences were observed in the ratios of colonies expressing βIII tubulin, nestin, or octamer (Oct)3/4. Four weeks after transplantation into the cochleae of neonatal mice, the number of surviving transplants of TTF-derived iPS cells generated by retroviral infection was significantly higher than those of MEF-derived iPS cells generated by plasmid transfection. Teratoma formation was identified in one of five cochleae transplanted with TTF-derived iPS cells. However, no significant differences were found in the cell-proliferation activity or the extent of differentiation into mature neurons among the cell lines. These findings emphasize the necessity of selecting appropriate iPS cell lines and developing methods to eliminate undifferentiated cells after neural induction, in order to establish safe iPS cell-based therapy for the inner ear.
- 2012-02-02
論文 | ランダム
- 有機導電性化合物の合成 (機能性炭素化合物に関する研究)
- ヌクレオシドおよびヘキソピラノシドの位置選択的炭素鎖延長反応
- アゾジカルボン酸ジエチルとトリフェニルホスフィンを用いる縮合反応
- 構造-音場連成系の固有モード感度解析手法の開発
- 計算力学的手法を使った騒音振動解析の現状と展望 (シミュレ-ション)