Force Measurement Enabling Precise Analysis by Dynamic Force Spectroscopy
スポンサーリンク
概要
- 論文の詳細を見る
Dynamic force spectroscopy (DFS) makes it possible to investigate specific interactions between two molecules such as ligand-receptor pairs at the single-molecule level. In the DFS method based on the Bell-Evans model, the unbinding force applied to a molecular bond is increased at a constant rate, and the force required to rupture the molecular bond is measured. By analyzing the relationship between the modal rupture force and the logarithm of the loading rate, microscopic potential barrier landscapes and the lifetimes of bonds can be obtained. However, the results obtained, for example, in the case of streptavidin/biotin complexes, have differed among previous studies and some results have been inconsistent with theoretical predictions. In this study, using an atomic force microscopy technique that enables the precise analysis of molecular interactions on the basis of DFS, we investigated the effect of the sampling rate on DFS analysis. The shape of rupture force histograms, for example, was significantly deformed at a sampling rate of 1 kHz in comparison with that of histograms obtained at 100 kHz, indicating the fundamental importance of ensuring suitable experimental conditions for further advances in the DFS method.
論文 | ランダム
- ベシ-ム S.ハキ-ム著,佐藤次高監訳「イスラ-ム都市--アラブのまちづくりの原理」
- 自由民主党衆議院議員 伊藤達也--権限と財源を地方に委譲し、『地方分権庁』を創設すべき (「地方再生」に向けて)
- 『財界人』トップインタビュー 今後の金融行政はどうなるのか!? 金融改革の行方 金融担当大臣 伊藤達也
- 海洋によって育まれた地球環境
- 地方公務員に国籍条項は必要か?--橋本大二郎高知県知事に聞く(インタビュ-)