The Isolation of Photoactive Chloroplasts from Leaves of the Mangrove, Kondelio condel Druce
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概要
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A method for isolating photoactive chloroplasts from mangrove leaves was investigated. Chloroplasts with the Hill reaction activity(photoreduction of ferricyanide) were isolated from the leaves of a mangrove plant, Kandelia candel Druce, by employing PEG in the grinding medium. Some characteristics of these chloroplasts are presented in this paper. Grinding medium for isolating photoactive chloroplasts is consisted of 1M sucrose,10%(W/V) PEG 4000, 0.025 M sodium iso-ascorbate, 0.05 M magnesium chloride and 0.05 M histidine buffered at pH 6.5 with HCI. Chloroplasts isolated without PEG reduces ferricyanide considerably in the dark. However, ferricyanide reduction in the dark was decreased by addition of PEG in the grinding medium. A possibility of tannins as the reductant is examined.
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