Lactobacillus gasseri の生産するバクテリオシンの食品利用へ向けた検討
スポンサーリンク
概要
- 論文の詳細を見る
Bacteriocins are natural antibacterial peptides ribosomally biosynthesized by bacteria. Gassericin T which is a bacteriocin produced by many strains of Lactobacillus gasseri has a broad antibacterial spectrum against food spoilage and pathogenic Gram-positive bacteria; therefore, the bacteriocin and its producers are predicted for use as safe food preservatives. However, probiotic Lb. gasseri strains show poor growth and minimally produce gassericin T in food-gradenatural media such as reconstituted cheese whey, unlike in MRS broth. The growth of Lb. gasseri in reconstituted cheese whey was improved by adding proteose peptone as a nutrient factor. The production of gassericin T in MRS broth was specifically inhibited by adding divalent metal cations, and then the inhibition was removed using a chelator of divalent cations, trisodium citrate dihydrate. The production of gassericin T in reconstituted cheese whey was also restored by adding trisodium citrate dihydrate, and the activity was enhanced by a surfactant, Tween 80. In addition, trisodium citrate dihydrate led to over-production of and synergistic antibacterial effect with gassericin T. In this study, we developed a cheese whey-based medium containing proteose peptone, trisodium citrate dihydrate and Tween 80 for gassericin T production. The developed food-grade medium may contribute to the effective use of some bacteriocins from probiotic lactic acid bacteria for biopreservation of foods.
- 2012-02-01
論文 | ランダム
- 調査データファイル(第97回)やむをえず派遣として働く人が急増 派遣労働者対策の根幹は正社員への道づくり
- 調査データファイル(第96回)日本の衰退が現実味を帯びてきた今、企業は何をすべきか 企業体質強化のカギは従業員への利益還元
- 調査データファイル(第95回)地方企業に見る若年社員戦力化のポイント 若者教育のキーワードは 叱る、鍛える、評価する
- 調査データファイル(第94回)職能・職責を重視した人事システムの必要性 産業特性と職務に則した人事施策が技術革新を支える
- 調査データファイル(第93回)デンマーク・オランダモデルに見る労働市場の改善策 適正雇用と格差是正が派遣問題を解決に導く