General Anesthetics Inhibit Erythropoietin Induction under Hypoxic Conditions in the Mouse Brain.
スポンサーリンク
概要
- 論文の詳細を見る
[Background]: Erythropoietin (EPO), originally identified as a hematopoietic growth factor produced in the kidney and fetal liver, is also endogenously expressed in the central nervous system (CNS). EPO in the CNS, mainly produced in astrocytes, is induced under hypoxic conditions in a hypoxia-inducible factor (HIF)-dependent manner and plays a dominant role in neuroprotection and neurogenesis. We investigated the effect of general anesthetics on EPO expression in the mouse brain and primary cultured astrocytes. [Methodology/Principal Findings]: BALB/c mice were exposed to 10% oxygen with isoflurane at various concentrations (0.10–1.0%). Expression of EPO mRNA in the brain was studied, and the effects of sevoflurane, halothane, nitrous oxide, pentobarbital, ketamine, and propofol were investigated. In addition, expression of HIF-2α protein was studied by immunoblotting. Hypoxia-induced EPO mRNA expression in the brain was significantly suppressed by isoflurane in a concentration-dependent manner. A similar effect was confirmed for all other general anesthetics. Hypoxia-inducible expression of HIF-2α protein was also significantly suppressed with isoflurane. In the experiments using primary cultured astrocytes, isoflurane, pentobarbital, and ketamine suppressed hypoxia-inducible expression of HIF-2α protein and EPO mRNA. [Conclusions/Significance]: Taken together, our results indicate that general anesthetics suppress activation of HIF-2 and inhibit hypoxia-induced EPO upregulation in the mouse brain through a direct effect on astrocytes.
論文 | ランダム
- 海洋埋立地の緑化
- 港湾緑化と造林技術 とくにヘドロ埋立地の特質と緑化対策について
- ポプラの栽培実績
- ポプラ栽培事業の発展と東海地方のポプラ普及の現況について
- Aspergillus japonicusの生産するヒドロキシシナミック酸エステル加水分解酵素の諸性質およびワイン醸造への利用〔微生物のヒドロキシシナミック酸エステル加水分解酵素〕