DNA Sequence of Cutinase Gene Isolated from Spilocaea oleagina as Determined by PCR
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概要
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[Synopsis] In this study, partial cutinase genes were cloned from Spilocaea oleagina and four different formae specials of Fusarium oxysporum (lycopersici, melonis, spinaciae and fragariae) by using PCR method. The reported amino acid sequences were aligned with DNA Data Bank of Japan (DDBJ), and highly conserved amino acid regions were selected to construct specific primers of cutinase genes. Chromosomal DNA of S. oleagina was isolated and used for template of PCR to amplify cutinase genes. Five DNA fragments were amplified by PCR with those primers and the amplified DNA fragments were cloned into T-vector for southern blot analysis and for determination of DNA sequences. The SO5 fragment amplified from S. oleagina was used for probe to analyze the cutinase gene of other 4 types of Fusarium oxysporum. SO5 fragment was hybridized to DNA fragments amplified from 4 formae speciales of Fusarium with the same primers indicating that similar sequences were conserved for cutinase in spite of their systematical distance from S. oleagina. The S05 DNA sequences cloned from S. oleagina were partially determined.
- 近畿大学農学総合研究所の論文
- 1998-03-00
近畿大学農学総合研究所 | 論文
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