The ROS scavenging and renal protective effects of pH-responsive nitroxide radical-containing nanoparticles
スポンサーリンク
概要
- 論文の詳細を見る
The ultimate objective of nanoparticle-based therapy is to functionalize nanomedicines in a micro-disease environment without any side effects. Here, we reveal that our pH-responsive nitroxide radical-containing nanoparticles (RNPpH) disintegrate within the renal acidic lesion and act as scavengers of reactive oxygen species (ROS), leading to a relief of acute kidney injury (AKI). RNPpH was prepared using amphiphilic block copolymers possessing 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO) moieties via amine linkage as a side chain of the hydrophobic segment. The self-assembled RNPpH disintegrated at pH below 7.0 because of a protonation of the amino groups in the hydrophobic core of the nanoparticles, thereby resulting in an improvement in ROS scavenging activity. Using a renal ischemia-reperfusion AKI model in mice, the therapeutic effect of RNPpH on ROS damage was evaluated. Unlike the RNP without pH-triggered disintegration (RNPNon−pH), the RNPpH showed extremely high ROS scavenging activity and renal protective effects. It is interesting to note that the side effect of nitroxide radicals was markedly suppressed due to the compartmentalization of nitroxide radicals in the core of RNPpH in untargeted area. The morphology changes in RNPpH were confirmed by analyzing electron spin resonance spectra, and these findings provide the evidence of the real therapeutic effect of the environment-sensitive specific disintegration of nanoparticles in vivo.
論文 | ランダム
- 議会改革リポート 変わるか!地方議会(103)自治基本条例を起点に、行政評価や議会報告会を実施--長野県飯田市議会
- 新刊案内 飯田市歴史研究所編『松尾大森本の家と周辺の社会』
- 長野県・飯田市中心市街地活性化基本計画の概要
- 特集 第25回「教育奨励賞」受賞校 努力賞 キャリア教育通して生き方を探す(24)長野県飯田市立飯田西中学校
- 環境モデル都市「飯田市」の挑戦 (特集 地球温暖化に対応した国土づくり)