Self-assemble gene delivery system for molecular targeting using nucleic acid aptamer.
スポンサーリンク
概要
- 論文の詳細を見る
We have developed a novel vector constructed with pDNA, polyethylenimine (PEI), and mucin 1 (MUC1) aptamer for tumor-targeted gene delivery. The MUC1 aptamer and non-specific aptamer were employed to coat the pDNA/PEI complexes electrostatically and stable nanoparticles were formed. The addition of a non-specific aptamer to the pDNA/PEI complex decreased gene expression in the human lung cancer cell line, A549 cells expressing MUC1 regularly. At the same time, the pDNA/PEI/MUC1 aptamer complex showed higher gene expression than pDNA/PEI/non-specific aptamer complex. Furthermore, the pDNA/PEI/MUC1 aptamer complex showed markedly high gene expression in tumor-bearing mice; thus, pDNA/PEI/MUC1 aptamer complexes are useful as a tumor-targeted gene delivery system with high transfection efficiency.
- 2011-10-01
論文 | ランダム
- AE法によるコア・コンクリート供試体の損傷度の同定
- 書評 岩間英夫:日本の産業地域社会形成
- 日本における工業都市化の内部構造とその発達過程
- 川崎・鶴見と尼崎の比較からみた総合工業地域社会の内部構造とその発達過程
- 尼崎と日立・八幡などの比較からみた工業地域社会の内部構造とその発達過程