Dynamic epigenetic regulation of OsLTP2 gene expression during seed development of rice
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We found wound- and pathogen-responsive ex-pression of OsLTP2 gene in leaves of various Tos17 mutants, whose genome were randomly inserted by retrotransposons, and transgenic rice plants during study of stress responses of rice. We assumed that DNA methylation state of the OsLTP2 promoter was altered in a Tos17 mutant line ND7315, because DNA sequence of the OsLTP2 was completely identical to that in wild type, Nipponbare. It was revealed that the OsLTP2 promoter of Nipponbare was highly methylated, whereas the promoter of ND7315 was completely demethylated, by bisulfite sequencing. These results strongly suggest that the demethyla-tion of OsLTP2 promoter cause the alteration of OsLTP2 ex-pression pattern in Tos17 mutants. In Nipponbare, while no expression of OsLTP2 was observed in vegetative organs and spicules at immediately after flowering, strong expression of OsLTP2 was observed in spicules at 5-15 days after flowering. To examine whether dynamic epigenetic alteration of the pro-moter region regulates the stage specific expression of OsLTP2 or not, we investigated the methylation state of OsLTP2 pro-moter at various stages of spicules and seeds. Prominent alter-ation of DNA methylation level in OsLTP2 promoter was ob-served and the level of DNA methylation was greatly correlated with a level of OsLTP2 expression. The methylation of OsLTP2 promoter region was mostly disappeared in 10-15 days after flowering (DAF) developing seeds, and then it was regenerated to original methylation pattern in embryos of dry seeds. These results suggest that the epigenetic alteration of OsLTP2 promoter was programmed in cells of developing seeds.
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- 平成22年度昭和大学公開講座--昭和大学歯科病院 暮らしと健康--健康と若さは口元から