Ubiquitin chains in the Dsk2 UBL domain mediate Dsk2 stability and protein degradation in yeast
スポンサーリンク
概要
- 論文の詳細を見る
Ubiquitin-like (UBL)-ubiquitin-associated (UBA) proteins, including Dsk2 and Rad23, act as delivery factors that target polyubiquitinated substrates to the proteasome. We report here that the Dsk2 UBL domain is ubiquitinated in yeast cells and that Dsk2 ubiquitination of the UBL domain is involved in Dsk2 stability, depending on the Dsk2 UBA domain. Also, Dsk2 lacking ubiquitin chains impaired ubiquitin-dependent protein degradation and decreased the interaction of Dsk2 with polyubiquitinated proteins in cells. Moreover, Dsk2 ubiquitination affected ability to restore the temperature-sensitive growth defect of dsk2 Delta. These results indicate that ubiquitination in the UBL domain of Dsk2 has in vivo functions in the ubiquitin-proteasome pathway in yeast.
- 2011-08-05
論文 | ランダム
- 座談会 地球温暖化の科学--IPCCの過去・現在・未来 (特集 地球温暖化問題への取組み--国立環境研究所)
- NiOを用いたスピンバルブ膜における積層構造の影響
- 108)徐脈頻脈症候群を呈した続発性アミロイドーシスの1例(第202回日本循環器学会関東甲信越地方会)
- 86)ホルター心電図装着中に急死した異型狭心症の1症例(第201回日本循環器学会関東甲信越地方会)
- 講演「生物学と心理学」(生物学と心理学の対話)